Modified Medium for the Rapid Growth of Mycobacterium Tuberculosis

The growth of Mycobacterium tuberculosis and Mycobacterium johnei in a modification of Dubos medium.

The success achieved in growing Mycobacterium tuberculosis in liquid medium (Dubos & Davis, 1946) has aroused interest in the possibility of growing M . johnei in this fluid or similar media, modified to contain the essential growth-promoting substances for these organisms. So far no success has been achieved (Davis & Dubos, 1948; Taylor, 1950). The combination of Tween 80 and bovine albumin fr...

A medium for the rapid cultivation of Mycobacterium avium.

The search for chemotherapeutic agents effective against the tubercle bacillus has brought into sharp focus the slow rate of growth of the organism. Dubos (1945), Davis and Dubos (1947), and Dubos and Middlebrook (1948) developed a liquid medium containing sorbitan monooleate ("tween 80") and albumin which supported rapid and uniform growth of tubercle bacilli. However, Long, as far back as 191...

The Effect of Penicillin on the Growth of Mycobacterium tuberculosis in Dubos' Medium.

The in vitro study of antibiotics for activity against Mycobacterium tuberculosis has been hampered by the lack of a convenient method for rapid cultivation of the organism. In the case of penicillin, instability of the antibiotic in solution presents an additional handicap. Abraham et al. (1941) reported that 1,000fold the amount of penicillin adequate to produce complete inhibition of a strai...

RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION

We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...

Minor Contribution of inhA-15 Mutations to the Rapid Detection of Isoniazid Resistance in Mycobacterium Tuberculosis Isolates